alicator ligation independent cloning (lic) and expression kit (Thermo Fisher)
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alicator ligation independent cloning (lic) and expression kit
Alicator Ligation Independent Cloning (Lic) And Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ligation+independent+cloning+(lic)+kit/alicator+ligation+independent+cloning+and+expression+system/pm40181138-38-7-9
Average 90 stars, based on 1 article reviews
Alicator Ligation Independent Cloning (Lic) And Expression Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ligation+independent+cloning+(lic)+kit/alicator+ligation+independent+cloning+and+expression+system/pm40181138-38-7-9
Average 90 stars, based on 1 article reviews
alicator ligation independent cloning (lic) and expression kit - by Bioz Stars,
2026-09
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Ligation:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Cloning:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Amplification:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Clone Assay:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Plasmid Preparation:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Expressing:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into Sequencing:Article Title: Engineered Human Monoclonal scFv to Receptor Binding Domain of Ebolavirus Article Snippet: For production of cell-penetrating HuscFvs (transbodies) and large-scale production of the transbodies and the original HuscFvs, the huscfvs in the pCANTAB 5E phagemids were subcloned into Article Title: New biochemical insights of CCA enzyme role in tRNA maturation and an efficient method to synthesize the 3'-amino-tailed tRNA. Article Snippet: The maturation of tRNA and its quality control is crucial for aminoacylation and protein synthesis.. The CCA enzyme, also known as tRNA nucleotidyltransferase, catalyzes the addition or repair of CCA at the 30terminus of tRNAs to facilitate aminoacylation.. Structural studies of CCA enzyme in complex with ATP and CTP suggested that adding CCA at the 30-terminus of tRNAs is a sequential process [1e4]. Article Title: Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger. Article Snippet: Chem. (2023) 299(8) 105003 NRRL3_02586) or pLATE52 (NRRL3_00837) vectors by Article Title: Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies. Article Snippet: Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l homepage: ht tp : / /www.e lsev ie r .com/ locate / i jb iomac Characterization of active/binding site residues of peptidyl-tRNA hydrolase using biophysical and computational studies Rajkumar Kulandaisamy a, Tushar Kushwaha a, Vikas Kumar a, Soumya De b, Saroj Kumar a, Santosh Kumar Upadhyay c, Manoj Kumar a, Krishna K. Inampudi a,⁎ a Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India b School of Bio Science, Indian Institute of Technology, Kharagpur, West Bengal 721302, India c CSIR-Institute of Genomics & Integrative Biology, New Delhi 110025, India ⁎ Corresponding author at: Department of Biophysic Sciences, New Delhi 110 029, India.. E-mail address: krishna.inampudi@aiims.edu (K.K.. Ina https://doi.org/10.1016/j.ijbiomac.2020.05.133 0141-8130/© 2020 Elsevier B.V. All rights reserved. a b s t r a c t a r t i c l e i n f o Article history: Received 10 December 2019 Received in revised form 9 April 2020 Accepted 17 May 2020 Available online 20 May 2020 Keywords: Mycobacterium tuberculosis (M. tb) Escherichia coli (E. coli) Peptidyl tRNA Peptidyl tRNA hydrolase (PtH) Peptidyl tRNA analogue Isothermal titration calorimetry (ITC) Fourier transform infra-red (FTIR) spectroscopy Circular dichroism (CD) spectroscopy and modelling studies All mRNAs cannot be translated into full-length proteins due to ribosome-stalling that leads to release of peptidyl-tRNA which can be lethal for bacterial survival. Article Title: Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity Article Snippet: For generation of cell penetrable HuscFvs (transbodies) and large-scale production of transbodies and the original HuscFvs, the huscfvs in the pCANTAB5E phagemids ( ) were subcloned into |